Caltech Analytical Resources

 

Caltech High Performance Cluster:  a large network of ordinary computers that work together to solve challenging problems. It is a coordinated group of server computers running the CentOS Linux 7. It is used for high-performance computing applications that range from seismology to climate models to genomic analysis. (http://www.hpc.caltech.edu/)

Functional Genomics Center:  Center develops and disseminates experimental and computational methods for functional genomics. These include RNA transcript expression (RNA-seq) from traditional samples, small pools of cells and single cells; genome-wide transcription regulatory protein occupancy (ChIP-seq of all kinds); long distance chromosomal interactions; and various assays of regulatory elements.

Jacobs Genetics and Genomics Laboratory:   Provides genomic support to the Caltech community, with an emphasis on high throughput sequencing and microarray analysis. (http://mmjggl.caltech.edu)

Proteome Exploration Laboratory:  The Proteome Exploration Laboratory provides both scientific and technical expertise and state-of-the-art high performance and ultrahigh performance liquid chromatography and tandem mass spectrometric instrumentation. (http://beckmaninstitute.caltech.edu/pel.shtml)

  Transmission Electron Microscopy center:  Provides advice, training and access to high-end instrumentation that Caltech researchers need to perform state-of-the-art biological and/or cryogenic TEM, and seeks to further develop these technologies to better meet research objectives. (http://beckmaninstitute.caltech.edu/tem.shtml)

  Biological Imaging Facility:  The BIF has 6 microscopes. Five are confocal microscopes for optical sectioning of fluorescently labeled samples. Of these, 4 are laser scanning confocal microscopes and one is a Spinning Disc confocal for high speed imaging. Three of the laser scanning confocal microscopes have multiphoton/two-photon lasers (MP, a category of nonlinear optics, NLO) which allow for deeper imaging of tissues. The sixth microscope is a wide field deconvolution microscope for high throughput fluorescence imaging. (http://beckmaninstitute.caltech.edu/birc.shtml).

  Flow Cytometry/Cell Sorting Facility:  The facility is currently equipped with two cell sorters: BD FACSAria IIu cell sorter, capable of analyzing at least 9 colors utilizing 3 lasers (407nm, 488nm, & 633nm), and of carrying out 4-way sorting up to 10,000 cells per second with reliable efficiency and recovery, or 1-way sorting for single-cell cloning into various cell culture plate configurations and an iCyt Mission Technology Reflection (5-laser/9color (UV, 405, 488, 561, and 633nm) cell sorter with one Highly Automated Parallel Sorting (HAPS) module. (http://beckmaninstitute.caltech.edu/fccsf.shtml)