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Funded by the National Science Foundation via the
Microbial Interactions and Processes (MIP) program.
For more on the project, see the Executive Summary.

Examination of diverse anaerobic methane-oxidizing Archaea and associated syntrophic relationships using high resolution
molecular and isotopic methods

def: anaerobic – in the absence of oxygen.

(an anaerobe is an organism, such as a bacterium, that can live in the absence of atmospheric oxygen)

def: methane – An odorless, colorless, flammable gas.

Why it is important?
Why do we study it?

The occurrence and biological regulation of net methane oxidation under anoxic conditions in the marine environment has been recognized as a significant process, but the identity of the microorganisms mediating this process has, until recently, remained elusive.

def: methane-oxidizing – getting energy from the oxidation of methane
            (some bacteria can do this).

def: oxidation - Any chemical reaction in which a material gives up electrons.  Burning is an example of rapid oxidation; rusting is an example of slow oxidation.

def: Archaea – single-celled prokaryotes genetically distinct from bacteria
            (many thrive in extreme environments, such as hydrothermal vents
            and hypersaline lakes).

def: syntrophic – when two distinct species interact in a favorable way.

def: high resolution molecular and isotopic methods

Sample collection: see photos of push cores
Geochemical characterization:
Molecular Characterization:
Visualization of gene expression using fluorescence in-situ hybridization microscopy.

Goals of the Expedition

To identify and characterize microbes responsible for methane production and consumption in coastal marine sediments.

Task 1 – Characterization of microorganisms involved in the production, consumption and transfer of methane-derived carbon within marine methane seeps.

Task 2 – Constraining thermodynamic models of the anaerobic oxidation of methane by identifying the spatial arrangement of active cells within natural aggregations

 


Goals  |  Summary  |  Microbes and Methane  |  Where and Why
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